





The CChip disposable hemocytometer is used for manual cell counting. In research settings, it helps to determine cell concentration prior to cell passage, or to assess cell viability following drug treatment; cell counting solution is essential. Obtaining accurate cell counts can be critical when seeding cells for propagation and experimentation.


Accuracy of manual counts with a hemocytometer depends on:

 Accurate mixing of the sample (no bubbles)
 Number of cells counted (practical: 200500 per 0.1 mm3)


In a conventional glass hemocytometer, improper fitting of the chamber and coverslip changes the volume of the sample introduced into the chamber. CChip¡¯s chamber with integrated coverslip solves the problem. It is a good standard practice to use a disposable hemocytometer for clinical labs and laboratories dealing with infectious materials. It is safer.


DHCN01, CChip disposable hemacytometer, has two precision engineered individual counting chambers (100 mm depth) with integrated cover slip. The chamber has ports for sample loading. Neubauer Improved (NI) grid pattern is embedded in each chamber. Each grid pattern has nine large squares each measuring 1 x 1 mm and a total counting area of 3 x 3 mm.



Applications



 Mammalian cell
 Blood analysis (Hematology): Blood cell counting
 Cell culture: Cell concentration measurement, Cell viability

Outer Size

Width 25mm x Length 75mm x Thickness 1.6mm

Chamber Depth

0.1 mm

Chamber Volume

10 µl




Calculation



The full grid on a hemocytometer contains 9 squares, each of which is 1 mm square. Large cells are counted using the four large corner squares (and the middle one). If you use a dense suspension of small cells, the four 1/25 sq. mm corners plus the middle square in the central square are used.
The grid consists of 9 large squares, each measuring 1 x 1 mm, and a chamber depth of 0.1 mm. Each square has a total volume of 0.1 mm^{3} or 10^{4}cm^{3}. The central square is divided into 25 small squares with triple lines and four corner squares are divided into 16 small squares.
Each large square has a surface area of 1.0mm^{2}, and the depth of the chamber is 0.1mm. As there are 1000 mm^{3} per ml, each large square represents a volume of 0.0001ml, so that it is equal to 1/ 0.0001ml = 10,000 = (10^{4})



Cells per ml = Average count per square x dilution factor x 10,000



Useful References:







Contact Point


