The C-Chip disposable hemocytometer is used for manual cell counting. In research settings, it helps to determine cell concentration prior to cell passage, or to assess cell viability following drug treatment; cell counting solution is essential. Obtaining accurate cell counts can be critical when seeding cells for propagation and experimentation.
Accuracy of manual counts with a hemocytometer depends on:
In a conventional glass hemocytometer, improper fitting of the chamber and coverslip changes the volume of the sample introduced into the chamber. C-Chip’s chamber with integrated coverslip solves the problem. It is a good standard practice to use a disposable hemocytometer for clinical labs and laboratories dealing with infectious materials. It is safer.
DHC-N01, C-Chip disposable hemacytometer, has two precision engineered individual counting chambers (100 mm depth) with integrated cover slip. The chamber has ports for sample loading. Neubauer Improved (NI) grid pattern is embedded in each chamber. Each grid pattern has nine large squares each measuring 1 x 1 mm and a total counting area of 3 x 3 mm.
The full grid on a hemocytometer contains 9 squares, each of which is 1 mm square. Large cells are counted using the four large corner squares (and the middle one). If you use a dense suspension of small cells, the four 1/25 sq. mm corners plus the middle square in the central square are used.
The grid consists of 9 large squares, each measuring 1 x 1 mm, and a chamber depth of 0.1 mm. Each square has a total volume of 0.1 mm3 or 10-4cm3. The central square is divided into 25 small squares with triple lines and four corner squares are divided into 16 small squares.
Each large square has a surface area of 1.0mm2, and the depth of the chamber is 0.1mm. As there are 1000 mm3 per ml, each large square represents a volume of 0.0001ml, so that it is equal to 1/ 0.0001ml = 10,000 = (104)
Cells per ml = Average count per square x dilution factor x 10,000
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