Manual microscopic cell counting in a Fuchs-Rosenthal (FR) chamber has been the gold standard for quantification of leukocytes in cerebrospinal fluid (CSF). An excellent correlation between cell counts obtained with the C-Chip chamber and FR chamber was observed. (“Determination of leukocyte counts in cerebrospinal fluid with a disposable plastic hemocytometer” Link to http://www.ncbi.nlm.nih.gov/pubmed/17847111 )
C-Chip DHC-F01 eliminates the risk of exposure to potentially infectious materials and the risk of improper fitting of the chamber and the cover slip, which improves the precision and repeatability.
The full grid on a hemocytometer contains 16 squares, each of which is 1 mm square. A dense suspension of small cells is 1/4 sq. mm.
Each large square has a surface area of 1.0mm2, and the depth of the chamber is 0.2mm. As there are 1000 mm3 per ml, each large square represents a volume of 0.0002ml, so that it is equal to 1/ 0.0002ml = 5,000. The volume factor is 5,000.
Cells per ml = Average count per square x dilution factor x 5,000
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