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DHC-B02 (Burker Turk)

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Product information

Product description

 

 

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Applications Cell counts in cerebrospinal fluid (CSF), rare cells

 
  • · Blood analysis(Hematology): Blood cell counting
  • · Cell culture: Cell concentration measurement, Cell viability test
  • · Microbiology : Fungal spores counting

 Outer Size​

 

 Width 25mm x Length 75mm x Thickness 1.6mm​

 

 Chamber Depth​

 

 0.1 mm​

 

 Chamber Volume​

 

 

10 µl

 

 

 

 

 

 

Calculation

 

The full grid on a hemocytometer contains 9 squares, each of which is 1 micro meter (mm) square.  Large cells are counted using the four large corner squares (and the middle one).

For a dense suspension of small calls, the four 1/25 sq. mm corners plus the middle square and the central square are used.

It consists of 9 large squares, each measuring 1 x 1 mm, with the depth of chamber of 0.1 mm.  Each square has a total volume of 0.1 mm3 or 10-4cm3.  The central square is divided into 25 small squares with triple lines and four corner squares divided into 16 small squares.

Each large square has a surface area of 1.0mm2, and a chamber depth of 0.1mm.  There are 1000 mm3 per ml, each large square represents a volume of 0.0001ml, that equates to 1/ 0.0001ml = 10,000 = (104).  The volume factor is 10,00

 


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Cells per ml = Average count per square x dilution factor x 10,000

 

 

 

 

 

 

 

 

 


 

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