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Outer Size
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Width 25mm x Length 75mm x Thickness 1.6mm
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Chamber Depth
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0.1 mm
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Chamber Volume
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The full grid on a hemocytometer contains 9 squares, each of which is 1 micro meter (mm) square. Large cells are counted using the four large corner squares (and the middle one). For a dense suspension of small calls, the four 1/25 sq. mm corners plus the middle square and the central square are used. It consists of 9 large squares, each measuring 1 x 1 mm, with the depth of chamber of 0.1 mm. Each square has a total volume of 0.1 mm3 or 10-4cm3. The central square is divided into 25 small squares with triple lines and four corner squares divided into 16 small squares. Each large square has a surface area of 1.0mm2, and a chamber depth of 0.1mm. There are 1000 mm3 per ml, each large square represents a volume of 0.0001ml, that equates to 1/ 0.0001ml = 10,000 = (104). The volume factor is 10,00 |
Cells per ml = Average count per square x dilution factor x 10,000
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